Part:BBa_K5425007

Short description

This circuit was designed to determine the threshold population at which the pLux promoter becomes active due to the binding of TetR and AHL. The threshold is influenced by several factors related to the components used in the circuit's design. The main purpose of this circuit is to assist in optimizing the design of the part. To monitor this activation, sfGFP was used as the reporter.

Introduction

This part fluoresces at a specific threshold population, providing insight into the performance and behavior of the components used in the construction of Part:BBa K5425004.

Sequence and Features

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1854
  
• 1000
  COMPATIBLE WITH RFC[1000]

Modelling and Characterization

Cloning and expression

All the sequences were sourced either from NCBI or the Parts Registry and then assembled in silico using SnapGene. For cloning, we used the part BBa_K5425004 which was previously cloned by us.

Fig:After induction with 2mM iptg and 200nm aTc